P-237: Chronic Alcoholism Induced Male Infertility :Impairment of Sperm Quality
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Abstract:
Background: Prolonged alcohol abuse in men can cause testosterone deficiency and shrinkage of the testicles (testicular atrophy). This can lead to impotence, sterility and ‘feminisation’. When alcohol is broken down in the body, it may also interfere with normal sperm structure and movement. This study was designed to determine the effects of administration of alcohol on sperm quality, sperm maturation and DNA damage Materials and Methods: Twenty adult male mice were divided in 2 groups: control sham (salin normal) and test group received ethanol (3 g/kg body weight as 25%, v/v) was given by gastric intubation daily for 45 days. After 45 days all mice were sacrificed and cauda epididymis were removed and placed in 1ml HTF+BSA 4mg/ ml and incubated for 30 min in Co2 incubator (5% Co2, 37°C) to allow the spermatozoa to swim out (p<0.05). The spermatozoal suspension was analyzed for sperm motility, concentration in the cauda epididymis, viability and sperm chromatin quality and DNA integrity was assessed by Aniline Blue and Acridine Orange staining following sperm sample. Results: This study showed that treatment with ethanol caused significant decrease in sperm concentration in cauda epididymis, motility, and viability, while abnormal sperms increased as compared to control. These changes were associated with significant increase in DNA damage and chromatin abnormality in the cauda epididymal spermatozoa as evidenced by Acridine Orange (AO) and Aniline Blue staining respectively (p<0.05) Conclusion: The present study demonstrates that chronic consumption of ethanol has toxic effect on spermatozoa and impairs fertility in male mice. Although it is hypothesized that chronic consumption of ethanol induced testicular injury with adverse effect on sperm production, sperm DNA integration and nuclear maturation.
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volume 6 issue 2
pages -
publication date 2012-09-01
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